644 OPR_R - TMPB UNL RD D ; TMPB = popped SS; read ES
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This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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Kleber Mendonça Filho's Oscar–nominated, BAFTA–nominated, Golden Globe–winning political crime thriller The Secret Agent is finally available to watch at home — and you absolutely should. Set mostly in the '70s during Brazil's military dictatorship, the film sees Civil War star Wagner Moura delivering a riveting performance as a man trying to evade persecution in his own country.
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